TABLE 2.

Retention times of aminoglycoside modification products after acetylation reactions

Aminoglycoside acetyltransferase	Retention time (min) of aminoglycoside modification product
	Tobramycin		Neomycin
	With acetyl-CoA	Without acetyl-CoA	With acetyl-CoA	Without acetyl-CoA
AAC(6′)-Iad	3.3	17.0	4.9	11.8
Positive controls
AAC(6′)	3.3	16.9	4.9	11.8
AAC(2′)	11.3	16.9	10.5	11.9
AAC(3)	4.4	16.9	6.7	11.9