Skip to main content
. 2014 Aug 5;13(9):4074–4090. doi: 10.1021/pr500437x

Figure 3.

Figure 3

Subcelullar localization in HeLa cells of NEK7, NEK9, TUBB, and PCNT (Pericentrin) throughout the cell cycle. Endogenous proteins were detected with primary antibodies against indicated proteins and revealed with Alexa Fluor 488 or Alexa Fluor 546 (Molecular Probes Inc.) secondary antibodies. The images were visualized by confocal fluorescence microscopy using an Operetta High Content Imaging System (PerkinElmer). PCNT was used to stain the centrosome. The nucleus was stained by Hoechst. The images were edited using Volocity Demo version 6.1.1 software (PerkinElmer). The images represent analyses of at least 25 cells in each cell cycle phase from three independent experiments, and all cells showed the localization pattern represented in the images. Magnitude: 40X. The scale bars are indicated. Short arrows denote spindle pole, long arrows indicate cytoplasmic bridge, and arrowheads denote centrosome staining.