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. 2014 Aug 5;13(9):4074–4090. doi: 10.1021/pr500437x

Figure 5.

Figure 5

Comparison of the interaction profile of human NEK6 and NEK7 and the chimeric constructs N6C7 and N7C6 using proteins identified by the yeast two-hybrid system. (A) The upper panel shows the interaction profile of NEK6, NEK7, N6C7, and N7C6 with NEK6 interacting proteins SNX26 (Sorting nexin 26), TRIP4 (Thyroid hormone receptor interactor 4), PTN (Pleiotrophin isoform CRAc), PRDX3 (Peroxiredoxin 3), and NEK9, as described by Meirelles et al.22 The lower panel shows the interaction profile of NEK6, NEK7, N6C7, and N7C6 with NEK7 interacting proteins SFRP4, TMTC4, PLEKHA8, ANKS1B, and NEK9 obtained by our yeast two-hybrid screen. The tests were performed in triplicate using growth selection by 3-amino-1,2,4-triazole (3-AT) gradient in minimal medium without tryptophan, leucine, and histidine. (B) A schematic representation of relative positions of NEK6 and NEK7 domains (as described by O’ Regan et al.12 and Meirelles et al.19) and N6C7 and N7C6 chimeric constructs is shown. The results of the interaction profiles are indicated as follows: + = strong growth; (+) = reduced growth; – = no growth. aa: amino acid residues.