Table 1. Human NEK7 Interacting Proteins Identified by the Yeast Two-Hybrid System Screensa.
| redundancy in libraryc |
|||||||
|---|---|---|---|---|---|---|---|
| protein interacting with Nek7b | gene | Uniprot accession | coded protein residues (retrieved/complete sequence) | FB | BM | L | growth scored |
| Beta-2-microglobulin | B2M | P61769 | 1–119/119 | 1 | 0 | 0 | 1/0 |
| Serine/threonine-protein kinase Nek9 | NEK9 | Q8TD19 | 764–976/979 | 3 | 3 | 2 | 4/0 |
| Regulator of G-protein signaling 2 | RGS2 | P41220 | 1–211/211 | 0 | 0 | 1 | 1/1 |
| CDK-activating kinase assembly factor MAT1 | MNAT1 | P51948 | 1–309/309 | 1 | 2 | 0 | 1/1 |
| Secreted frizzled-related protein 4 | SFRP4 | Q6FHJ7 | 1–346/346 | 0 | 4 | 0 | 3/0 |
| Cytochrome b-c1 complex subunit 9 | UQCR10 | Q9UDW1 | 1–63/63 | 0 | 1 | 0 | 1/0 |
| NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondria | NDUFS1 | P28331 | 451–727/727 | 0 | 2 | 0 | 1/0 |
| 5-Aminolevulinate synthase, nonspecific, mitochondrial | ALAS1 | P13196 | 94–640/640 | 0 | 1 | 0 | 1/0 |
| NFU1 iron-sulfur cluster scaffold homologue, mitochondrial | NFU1 | Q9UMS0 | 22–208/254 | 1 | 1 | 4 | 1/0 |
| Tubulin beta-2B chain | TUBB2B | Q9BVA1 | 247–430/445 | 0 | 0 | 1 | 1/1 |
| Hemoglobin subunit alpha | HBA1 | P69905 | 1–142/142 | 0 | 2 | 0 | 1/0 |
| Hemoglobin subunit beta | HBB | P68871 | 1–147/147 | 0 | 2 | 0 | 1/0 |
| Elongation factor 1-alpha 1 | EEF1A1 | P68104 | 340–432/462 | 0 | 1 | 0 | 1/0 |
| Elongation factor Tu, mitochondrial | TUFM | P49411 | 13–455/455 | 1 | 0 | 0 | 1/1 |
| Pleckstrin homology domain-containing family A member 8 isoform 2 | PLEKHA8 | Q96JA3 | 103–418/459 | 0 | 0 | 2 | 1/0 |
| Actin, cytoplasmic 2 | ACTG1 | P63261 | 187–375/375 | 0 | 0 | 3 | 1/1 |
| 40S ribosomal protein S6 | RPS6 | P62753 | 1–228/249 | 0 | 1 | 0 | 1/1 |
| Coiled-coil and C2 domain-containing protein 1A | CC2D1A | Q6P1N0 | 501–940/951 | 0 | 1 | 0 | 1/1 |
| 40S ribosomal protein S4, X isoform | RPS4X | P62701 | 5–263/263 | 0 | 2 | 0 | 1/1 |
| Ubiquitin-40S ribosomal protein S27a | RPS27A | P62979 | 1–156/156 | 0 | 2 | 0 | 1/0 |
| Ankyrin repeat and sterile alpha motif domain-containing protein 1B | ANKS1B | Q7Z6G8 | 6–426/426 | 0 | 1 | 0 | 3/0 |
| FCH and double SH3 domains protein 2 | FCHSD2 | O94868 | 627–740/740 | 1 | 0 | 0 | 1/1 |
| MAP7 domain-containing protein 1 | MAP7D1 | Q3KQU3 | 715–841/841 | 1 | 0 | 0 | 1/1 |
| Transmembrane and TPR repeat-containing protein 4 | TMTC4 | Q5T4D3 | 467–741/741 | 0 | 0 | 9 | 4/0 |
| Transmembrane 9 superfamily member 3 | TM9SF3 | Q9HD45 | 398–589/589 | 0 | 0 | 2 | 1/0 |
a
The proteins were selected from yeast cells under growth conditions in minimal medium without tryptophan, leucine, and histidine and containing 3-AT.
b
Results from BLASTX (GenBank).
c
Absolute number of sequences retrieved of each human library (FB: fetal brain, BM: bone marrow, L: leukocyte).
d
Growth test in yeast cells transfected with recovered sequences in the vector pACT2 and pBTM116K-NEK7/empty pBTM116KQ (concentration of 3AT = 5 mM).