FIG. 1.

Schematic representation of Tn2009, composed of the mega element inserted into a Tn916-like transposon. The structure of mega is boxed, and ORFs are indicated by gray arrows. Tn916 is indicated by a black bar, and white arrows represent the relevant ORFs. The exogenous sequence at the right end of Tn2009 is in gray. The flanking chromosome of S. pneumoniae is represented by a black line; striped arrows indicate ORFs, designed according to the homologous ORFs of strain R6. Short black arrows indicate the positions of the oligonucleotide primers used for PCR amplification and sequencing.