Figure 4.

Flow cytometry revealed that DHM induces cell apoptosis. (A and B) HepG2 and QGY7701 cells were treated with various DHM concentrations (0, 50 and 100 μM) for 24 h, and MHcc97L, Hepal-6 and HL7702 cells were treated with various DHM concentrations (0, 50 and 100 μM) for 48 h, and analyzed using the Annexin V staining method. (C and D) Terminal deoxynucleotidyl transferase dUTP nick end labeling assay was used to analyze the number of late-stage apoptotic cells following DHM treatment. The experiments were independently performed in triplicate. ^*P<0.05, ^**P<0.01 and ^***P<0.001, compared with the untreated (0 μm) control. DHM, dihydromyricetin.