FIG. 1.

Time-kill assay. The rates of survival of melanized versus nonmelanized C. neoformans strain ATCC 24067 and H99 after exposure to various concentrations of voriconazole or amphotericin for 2 h compared to those of fungal cells incubated in PBS. Values are averages ± the standard errors of the means for four measurements. P values were calculated by comparing melanized and nonmelanized cells by using the Student t test. ✽, P < 0.001. Similar results were obtained in three separate experiments.