FIG. 2.

Time-kill assay with melanins. The rates of survival of nonmelanized C. neoformans strain ATCC 24067 and H99 exposed to voriconazole or amphotericin B with or without preincubation of the antifungal agents with C. neoformans melanin or synthetic melanin were compared. Values are averages ± the standard errors of the means for four measurements. P values refer to differences between the rates of survival for yeast cells after preincubation of the antifungal agents with either type of melanin and those for cells not preincubated with melanin (control). ✽, P < 0.001 (Student t test). Similar results were obtained in two independent experiments.