FIG. 5.

Effect of amphotericin B and voriconazole on capsule growth. (A) C. neoformans strain H99 cells grown to logarithmic phase in SAB medium were transferred to PBS with 10% serum in the absence or presence of amphotericin B (0.067 times the MIC) or voriconazole (1 timers the MIC). Large capsules were seen with cells grown without antifungal agents (PBS) and voriconazole. The capsules of cells grown with amphotericin B were small. Representative images were taken after suspension of cells in India ink. Scale bar, 10 μm. (B) Cells were grown as in panel A but were subsequently transferred to fresh SAB medium or starved in PBS for 24 h and then incubated in 10% serum in PBS (□) or in the same medium containing voriconazole at 0.5 (▪) or 1 (░⃞) times the MIC. The cells were incubated overnight prior to determinations of capsule and cell size (n = 20 to 40 cells). No differences were seen between cells grown in either PBS or SAB medium with or without voriconazole. The experiment was repeated twice with similar results.