TABLE 4.
Phagocytic index for strains of C. neoformans grown with or without 0.5 times the MIC of voriconazolea
| Strain | Drugb MICb | Mean phagocytic index ± SD |
||
|---|---|---|---|---|
| Controlc | MAb 18B7 | Complementd | ||
| ATCC 24067 | - | 0.027 ± 0.01 | 3.6 ± 0.3 | 0.14 ± 0.07 |
| 0.5 | 0.034 ± 0.01 | 3.2 ± 0.4 | 0.16 ± 0.03 | |
| H99 | - | 0 | 3.5 ± 0.7 | 0.18 ± 0.10 |
| 0.5 | 0.08 ± 0.01 | 4.0 ± 0.4 | 0.18 ± 0.02 | |
a
Each value represents the results from three wells. The experiment was performed three times with similar results. The phagocytic index is the number of organisms engulfed divided by number of macrophages.
b
Indicates whether voriconazole was absent (-) or present at a concentration of 0.5 times the MIC for the specific strain.
c
The phagocytosis assay was performed in the absence of opsonins.
d
Complement from 20% FCS (not heat inactivated).