Table 2. Summary of genome assembly statistics from various assembly approaches.

	Sage-Grouse				Clark's Nutcracker
		Reference-guided				Reference-guided
	De novo	Coverage >1x	Coverage >2x	Coverage >5x	De novo	Coverage >1x	Coverage >2x	Coverage >5x
% N Bases	0.04	44.35	63.36	79.99	0.09	39.55	55.86	76.34
N50 - No Break	343	90,198,103	90,394,695	90,527,046	503	65,905,513	73,959,172	74,132,310
N50 - Break 500	–	12,125	4,447	1,804	–	13,369	6,765	2,409
Complete CEGs	0	12	0	0	4	76	20	1

The terms ‘no break’ and ‘break 500’ refer to whether or not contigs were broken up by deleting regions that contained stretches of 500 or more ambiguous (“N”) nucleotides, and CEGs refer to core eukaryotic genes.