Table 1.
Localization of Hoxb genes outside of CTs
| % signals outside of MMU11 CT
|
|||||
|---|---|---|---|---|---|
| Gene | Undiff. | Day 4 | Day 10 | TSA | TSA + 2 d |
| Hoxb1 (MAA) | 36 | 73 | 54 | 18 | 67 |
| Hoxb1 (pFa) | 15 | 65 | N/D | N/D | N/D |
| Hoxb9 (MAA) | 24 | 24 | 39 | N/D | N/D |
The percentage of Hoxb1 or Hoxb9 hybridization signals manually scored as being outside of the MMU11 CT by FISH to either MAA- or pFa-fixed nuclei from undifferentiated ES cells, and from cells after 4 and 10 d of differentiation. Undifferentiated cells that had been treated with TSA, and TSA-treated cells induced to differentiate with RA for 2 d, were also examined. N/D indicates not determined.