Table 2.

PCR primers and amplification conditions

	Gene/locus	Primers	Product size (bp)	PCR conditions
RT—PCR	Oct4	GGCGTTCTCTTTGGAAAGGTGTTC CTCGAACCACATCCTTCTCT	312	15 min 95°C (30 sec 95°C, 30 sec 55°C; 30 sec 72°C) × 30
	Gapdh	CTCAAGATTGTCAGCAATGCA CCTTCCACAATGCCAAAGTT	70	15 min 95°C (30 sec 95°C, 30 sec 61°C, 25 sec 72°C) × 25
	Hoxb1	CCATATCCTCCGCCGCAG CGGACTGGTCAGAGGCATC	450	15 min 95°C (30 sec 95°C, 30 sec 62°C, 40 sec 72°C) × 35
	Hoxb9	CAGGGAGGCTGTCCTGTCTAATC CTTCTCTAGCTCCAGCGTCTGG	176	15 min 95°C (30 sec 95°C, 30 sec 68°C, 20 sec 72°C) × 30
	Hoxb13	CTGGAACAGCCAGATGTGTT CCTGCTAAAGGTGTCATCTC	445	15 min 95°C (30 sec 95°C, 30 sec 57°C, 40 sec 72°C) × 35
	β-actin	GGTCAGAAGGACTCCTATGTGG TCTCAGCTGTGTGGTGAAG	475
ChIP	Hob1 r4 + promoter + initiation site	TTCCATGTCGCTCTCAGATG TAGGAAGGGGCTAGGGAGTG	172	15 min 95°C (30 sec 95°C, 30 sec 64°C, 20 sec 72°C) × 25
	Hoxb1 3′DR2	GACAGTGGGTCCTGGGGGT GGTGCTCAACAGGCTTGTTTGGA	110	15 min 95°C (30 sec 95°C, 30 sec 61°C, 20 sec 72°C) × 25
	Hoxb1 exon 1	CCATATCCTCCGCCGCAG TCTGACGAGCAAGGCGATTCCTTG	104
	Hoxb1 promoter	CCGCTTAGCCCATTGGCCT TAGGAAGGGGCTAGGGAGTG	80
	Hoxb9 promoter	TCCGAAAGCCCTCTGCAC GACATTCTCAGACATTATCCGCG	110
	Hoxb9 exon 1	GCACGCCCGAGTACAGTTTG CCTCTCTTTGTCCTCGCTTCCT	112