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. 2014 Sep 5;9(9):e107011. doi: 10.1371/journal.pone.0107011

Figure 3. Loss of Sirt3 impairs VEGF/VEGFR2 expression and angiogenesis in EPCs.

Figure 3

A. Cell proliferation was measured by MTT assay. The proliferative rate of EPCs was significantly reduced in cultured EPCs of Sirt3KO mice compared to that of WT mice (n = 4 mice, *p<0.05). B. EPC tube formation was significantly reduced in EPCs lack of Sirt3 when compared with control EPCs. Overexpression of Sirt3 significantly increased EPC tube formation (n = 4–6 mice, *p<0.05). C. BMC colony formation units. EPC colony formation was significantly reduced in Sirt3KO-EPCs when compared with WT-EPCs (n = 6 mice, *p<0.05). D and E. Western blot analysis showing that the basal levels of VEGF and VEGFR2 were significantly reduced in Sirt3KO-EPCs (n = 3 mice). Treatment of Sirt3KO-EPCs with NADPH oxidase inhibitor apocynin 200 and 400 µM or infection of Sirt3KO-EPCs with Ad-Sirt3 increased levels of VEGF and VEGFR2 expression (n =  3 mice). F. Western blot analysis showing that the basal levels of CXCR-4 expression were dramatic reduced in the Sirt3KO-EPCs (n = 3 mice).