Figure 4. Loss of Sirt3 in BMCs increases ROS formation in post-MI mice.

A and B. Western blot analysis revealing that BMC treatment resulted in a significant downregulation of p47^phox (A) and gp91^phox (B) expression compared to post-MI mice. Sirt3KO-BMC treatment did not inhibit expression of p47^phox and gp91^phox compared to BMC treatment. n = 6 mice, *p<0.05. C. Quantitative analysis of ROS formation by DHE staining revealing that BMC treatment resulted in a significant suppression of ROS formation compared to post-MI mice. Myocardial ROS formation was significantly higher in Sirt3KO-BMC treated mice than BMC treated mice. n = 5 mice; *p<0.05.