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. 2014 Sep 5;9(9):e106807. doi: 10.1371/journal.pone.0106807

Figure 3. IFN-γ and IL-17-producing CD4 T cell subsets proliferated in lymph nodes after rAg85B administration.

Figure 3

OVA-immunized (i.p., day0 and 14) and sensitized (5% aerosolized-OVA, day21 to 25) BALB/c mice were challenged with PBS or rAg85B protein (i.p. (100 µg; days 0 and 14) and i.n. (20 µg; days 21, 23, and 25)). At 24 h after the last OVA sensitization, mediastinal lymph nodes (MLNs) from naïve or OVA sensitized BALB/c mice treated with PBS or rAg85B, were harvested. MLNs were stimulated with ionomycin and PMA for 5 h, and with brefeldin A added in the last 3 h. Flow cytometry of stimulated MLNs from naïve (upper), PBS-treated (middle) and rAg85B protein-treated (lower) OVA-sensitized mice stained with specific antibodies indicated marker. Numbers in quadrants indicate percent of cells in each (A). Absolute numbers of various cell populations (above graphs) in lymph nodes (B, C). Data are representative of three independent experiments (*P<0.05, **P<0.01 compared with OVA control. error bars, s.d.; n = 6 mice).