Figure 1. Dietary Vitamin D₃ does not significantly alter epidermal thickening induced in response to UVB irradiation.

(a) Male SKH-1 mice were fed diets with increasing concentrations of Vitamin D₃ and irradiated thrice weekly for 25 weeks with UVB. The epidermal thickness from UV irradiated and unirradiated control mice are plotted. Error bars represent s.e.m. n = 15 UV exposed and n = 10 control unirradiated mice per treatment condition. (b) Representative images of irradiated and unirradiated skin after Haemotoxylin and Eosin (H&E) staining. Photos from SKH-1 mice fed standard or increasing concentration of vitamin D₃ chow were taken at a 10x magnification, scale bar  = 20 µm. (c) Ki67 staining in normal skin from irradiated and unirradiated skin obtained from mice fed diets with increasing concentration of Vitamin D₃ as indicated. Ki67 images were taken at a 20x magnification, scale bar  = 20 µm.