Skip to main content
. 2014 Sep 5;9(9):e107058. doi: 10.1371/journal.pone.0107058

Figure 3. Effects naringenin (NGEN) or naringenin complexed with copper (II) and 2,2′-bipyridine (NGENCuB) on MDA-MB-231 colony formation.

Figure 3

A. Clonogenic assay of untreated MDA-MB-231 cells (control) or treated with NGEN or NGENCuB. A photograph of Petri-dishes in a representative experiment is shown. B. Quantification of colony number. C. Quantification of colony size. D. Plate efficiency. Quantification of colony number and size was performed using Image J public domain software. Data represent means ± SD from three different experiments in triplicate. The results were compared using ANOVA, followed by a Tukey's post-hoc analysis. Asterisks represent *p≤0.05, **p≤0.01, ***p≤0.001 compared to control.