Figure 6. Effects of naringenin (NGEN) or naringenin complexed with copper (II) and 2,2′-bipyridine (NGENCuB) on MDA-MB-231 cell migration in Boyden chambers.

Control cells or compounds-treated cells were allowed to migrate towards lower insert chambers containing FBS as chemoattractant, for 22 hours. The morphology of cells in the different treatments migrating toward FBS is in the bottom panels. Positive control (C+) represents migrating cells without any treatment, negative control (C-) represents untreated cells migrating toward an FBS-free medium. Data represent mean ± SD of three independent assays in triplicate. The results were compared using ANOVA, followed by a Tukey's post-hoc analysis. Asterisks represent *p≤0.05, **p≤0.001 compared to control (C+).