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. 2014 Sep 5;9(9):e107058. doi: 10.1371/journal.pone.0107058

Figure 7. Effects of naringenin (NGEN) or naringenin complexed with copper (II) and 2,2′-bipyridine (NGENCuB) on activity of pro-MMP9 in MDA-MB-231 breast tumor cells.

Figure 7

A. Zymography in 1% gelatin-SDS-PAGE. Lane 1: molecular mass marker (M); lane 2: control cells; lanes 3–5: cells treated with NGEN; lane 6: control cells and lanes 7–9: cells treated with NGENCuB (n = 3; 10 µg of total protein was loaded in each lane). A photograph of a representative zymography gel is shown. B. pro-MMP9 concentrations were determined by the integrated optical density (IOD) obtained from the bands. Gels were analyzed by densitometry, and activity was expressed as arbitrary units (AU) and data were normalized in percentage compared to untreated control cell lysate. Data represent mean ± SD of three independent assays in triplicate. The results were compared using ANOVA, followed by a Bonferroni's multiple comparison test. Asterisk represents *p≤0.05.