Figure 2.

Restoration of miRNA-mediated translational repression of LIN-28 mutant MREs by synthetic let-7b siRNAs that carry compensatory mutations. (A) Potential base pairing between LIN-28 MREs (black) and endogenous let-7b or synthetic let-7b siRNAs (blue). Mutated nucleotides are shown in red. (B,C) HeLa cells were cotransfected with Renilla luciferase (RL) constructs bearing the indicated MREs in the 3′-UTR, along with firefly luciferase (FL) and with or without the indicated synthetic siRNAs (30 nM). Results shown are average values (with standard deviations) of normalized RL/FL activities obtained from three separate experiments.