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. 2004 May 15;18(10):1179–1186. doi: 10.1101/gad.1201204

Figure 2.

Figure 2.

Blot analysis of miRNA targets in transgenic Arabidopsis plants. RNA samples from vector-transformed (lanes 1,2) and suppressor-expressing transgenic Arabidopsis plants (lanes 3–12) were analyzed in duplicate by hybridization with DNA probes. Ethidium bromide-stained 28S rRNA is shown for each blot. Mobility positions of relevant RNA size standards (kb) are shown. (A) Accumulation of ARF8 (At 5 g 37020) mRNA. Mean relative accumulation (RA) of ARF8 mRNA in suppressor expressing plants was calculated relative to that in vector-transformed plants, normalized against the accumulation of the control TyrAT (At 2 g 20610) mRNA. (B) Accumulation of ARF10 (At 2 g 28350) mRNA. Mean RA of ARF10 mRNA was calculated as in A.(C) Expression of full-length SCL6-IV (At 4 g 00150) mRNA. The mean ratio of full-length SCL6-IV mRNA (a) to the cleavage product (b) is shown. (D) Expression of TyrAT (At 2 g 20610) mRNA. This blot was first used to analyze ARF10 expression, then stripped and reprobed.