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. 2014 Sep 5;9(9):e107072. doi: 10.1371/journal.pone.0107072

Figure 4. Effect of apigenin on LPS-induced proinflammatory cytokines mRNA expression in human THP-1-derived macrophages.

Figure 4

Cells were pretreated with different concentrations of apigenin (A, 6.25, 12.5,25 µM) for 2 h, then treated with LPS (100 ng/mL) for 24 hours. Total cellular RNA was isolated and reverse transcribed. The relative mRNA levels of IL-1β, IL-6, and TNF-α were detected by real-time RT-PCR as described under “Methods”. Values are mean ± S.E. of three independent experiments. Statistical significance relative to vehicle control, ##p<0.01; ###p<0.001; Statistical significance relative to LPS group, *p<0.05, **p<0.01, ***p<0.001. A. IL-1β; B. IL-6; C. TNF-α.