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. 2014 Sep 5;9(9):e107072. doi: 10.1371/journal.pone.0107072

Figure 11. Effect of apigenin on the mRNA and protein expression of ASC and NLRP3 in human THP-1-derived macrophages.

Figure 11

Cells were pretreated with apigenin (A, 6.25, 12.5, 25 µM) for 2 h and then treated with LPS (100 ng/mL) for 24 h. Total cellular RNA was isolated and reverse transcribed. The relative mRNA levels of PYCARD/ASC and NLRP3 were detected by real-time RT-PCR, as described under “METHODS”. Values are mean ± S.E. of three independent experiments. Statistical significance relative to LPS group, #p<0.05. A. The mRNA level of PYCARD/ASC; B. The mRNA levels of NLRP3. C. The protein levels of ASC and NLRP3 were detected by Western blot analysis as described under “EXPERIMENTAL PROCEDURES”. The representative images of ASC and NLRP3 are shown.