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. 2014 Sep 5;9(9):e107072. doi: 10.1371/journal.pone.0107072

Figure 13. Effects of apigenin on LPS-mediated mRNA stabilization in human THP-1-derived macrophages.

Figure 13

Cells were pretreated with apigenin (A, 25 µM) for 2 h and then treated with LPS (100 ng/mL) for 2 h, followed by treatment with actinomycin D (5 µg/ml). Total cellular RNA was isolated at 0, 0.5,1, 2, 4 and 6 h after actinomycin D treatment. The mRNA levels of IL-1β and IL-6 were determined by real-time RT-PCR as described under “METHODS”. Values are the means ± S.E. from three independent experiments. A. IL-1β; B. IL-6.