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. 2014 Sep 5;9(9):e107072. doi: 10.1371/journal.pone.0107072

Figure 16. Effect of apigenin on pro-inflammatory cytokine-induced activation of NF-κB.

Figure 16

Human 293 Cells were stably transfected with pGL4.32 [luc2P/NF-κB-RE/Hygro] luciferase reporter. Cells were pretreated with apigenin (6.25, 12.5,25 µM) for 2 h and then treated with human IL-1β (10 ng/mL) or TNF-α (10 ng/mL) for 4 h. The luciferase activity was detected as described under “METHODS”. Values are mean ± S.E. of three independent experiments. Statistical significance relative to vehicle control, ###p<0.001; Statistical significance relative to LPS group, **p<0.01, ***p<0.001. A. IL-1β; B. TNF-α.