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. 2014 Sep 5;9(9):e107176. doi: 10.1371/journal.pone.0107176

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© 2014 Mirajkar, Gebhart

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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In the Minimum Spanning Tree (MST) analysis, each circle represents a different sequence type (ST) as labeled, its size reflects the number of isolates and the color indicates the country of origin. The width of the lines reflects the genetic difference between two STs, wherein dark/heavy lines connect single locus variants (SLVs), light/thin lines connect double locus variants (DLVs) and dotted lines indicate the most likely connection between two STs differing by three or more loci. STs which were DLVs of at least one other ST were grouped together (shaded in grey).