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EHEC espF and U-espF are coordinately regulated. Equivalent amounts of (DNase-treated) RNA from EHEC strain 85-170 grown to mid-logarithmic phase in LB or DMEM were subjected to RT-PCR analysis by using gene-specific primers to espF and U-espF. Lanes 3 and 4 represent controls in which the reverse transcriptase was omitted. Lanes 5, 6, and 7 correspond to molecular weight markers, positive (EHEC chromosomal DNA template), and negative (no template) controls, respectively.
