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U-EspF can enhance EHEC-induced alteration of epithelial barrier function. The EHEC strain 85-170 was transformed with the pUC18 empty vector or vectors encoding full-length EHEC espf or U-espF. T84 cells were infected with these strains or treated with media alone (control), and TER measurements were recorded at 6 h postinfection (n > 10; asterisk denotes significant difference for U-espF compared to pUC18, where P is <0.0005; P > 0.1 for espF compared to pUC18).
