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. 2004 Jun;72(6):3410–3417. doi: 10.1128/IAI.72.6.3410-3417.2004

FIG. 3.

FIG. 3.

IgG antibodies from myosin-immunized mice do not react with medium proteins but do react with proteins in a T. cruzi lysate. Serum samples from five myosin-immunized or saline-immunized mice obtained 21 days postimmunization were pooled for use in a Western blot analysis. (A) LDNT homogenate (40, 20, and 10 μg) and Sigma myosin proteins (30, 3, and 0.3 μg) were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the same gel, stained with GelCode Blue or transferred to nitrocellulose, and blotted with a 1:200 dilution of sera from myosin-immunized mice (Myosin/CFA) or saline-immunized mice (Saline/CFA). (B) T. cruzi lysate (T; 10 μg) used for DTH assays and Sigma myosin (M; 0.3 μg) were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, stained with GelCode Blue or transferred to nitrocellulose, and blotted with a 1:200 dilution of sera from myosin-immunized mice (Myosin/CFA) or saline-immunized mice (Saline/CFA). The positions of molecular mass standards (in kilodaltons) are indicated at the left.