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. 2014 May 13;11(6):715–731. doi: 10.4161/rna.29143

Table 1. Mass spectrometric identification of TSR1 purification products (L.t).

  Protein category and accession number Annotation Trypanosoma brucei homolog Number of peptides e-value Purifications Reference(s) or source
Splicing factors
1 LmjF.07.0870 TSR1/RBSR1 Tb927.8.900 42,22 1E-30 NHP2-Lt, TSR1IP-Lt, U2AF35-Lt 29
2 LmjF.32.2200 U5–116K/Snu114 Tb11.01.7080 2 0.00000462 PRP19-Tb, Lsm3-Lt, SmB-Tb,65 SmD1-Tb,38 SmD3-Lt, 34 , 38 , 65
3 LmjF.33.3190 SmD2 Tb927.2.5850 1 0.000559 CPSF73, U1A-Lt, Lsm3-Lt, SmB-Tb,65 SmD1-Tb,38 SmD3-Lt, 65
4 LmjF.35.4460 SmF Tb09.211.1695 1 0.006573 Lsm3-Lt, SmB-Tb,65 SmD1-Tb,38 SmD3-Lt 65
5 LmjF.23.1550 SYF1 Tb927.5.1340 1 0.000471 PRP19-Tb, SmD1-Tb,38 SmD3-Lt, 34 , 38
6 LmjF.27.2100 TRRM1 Tb927.2.4710 2 0.000071 TSR1IP-Lt, U2AF65-Tb, U2AF35-Lt 28
7 LmjF.32.0400 ATP-dependent RNA helicase, putative Tb927.10.14550 8 0.0000683 CPSF73, SMN-Lt, NHP2-Lt, PRP19-Tb, U1A-Lt, Lsm3-Lt, SmD3-Lt, TSR1IP-Lt, U2AF65-Tb, U2AF35-Lt, SF1-Lt 34
8 LmjF.07.0340 ATP-dependent DEAD/H RNA helicase, putative Tb927.8.1510 2 0.000302 CPSF73, SMN-Lt, U1A-Lt, Lsm3-Lt, SmD3-Lt, TSR1IP-Lt, U2AF35-Lt, SF1-Lt 34
9 LmjF.35.3100 ATP-dependent RNA helicase, putative Tb09.211.3510 1 0.000318 U1A-Lt, Lsm3-Lt, U2AF35-Lt, SF1-Lt 34
mRNA metabolism
10 LmjF.35.5040 PABP1 Tb09.211.0930 12,18 3.41E-09 NHP2-Lt, U1A-Lt, SmB-Tb,65 SmD1-Tb,38 SmD3-Lt, TSR1IP-Lt, U2AF35-Lt 82 , 83
11 LmjF.35.4130 PABP2 Tb09.211.2150 19,25 1.07E-11 NHP2-Lt, PRP19-Tb, SmB-Tb,65 SmD1-Tb,38 SmD3-Lt, TSR1IP-Lt, U2AF35-Lt 84
12 LmjF.25.0080 PABP ** 6,9 7.22E-12 NHP2-Lt, SmB-Tb,65 SmD1-Tb,38 SmD3-Lt, TSR1IP-Lt, U2AF35-Lt GeneDB
13 LmjF.35.2200 DRBD2 Tb09.211.4540 5 0.0000665 NHP2-Lt, TSR1IP-Lt, U2AF35-Lt 85
14 LmjF.25.0500 UBP2 Tb11.03.0580 1 0.000466   86 - 88
15 LmjF.17.0550 RBP23 Tb927.10.11270 1 0.001262   46 , 85
16 LmjF.27.1110 MRP1 Tb11.55.0009 4 0.000000627 NHP2-Lt, U2AF35-Lt 49 , 50
17 LmjF.09.1120 MRP2 Tb11.01.4860 3 0.000123 TSR1IP-Lt, U2AF35-Lt 49 , 89
18 LmjF.13.0450 Alba2 Tb11.02.2030 1 0.00752   47
19 LmjF.25.0540 RAP55 Tb11.03.0530 1 0.009507 Lsm3-Lt, SmD3-Lt, SF1-Lt 34
20 LmjF.35.0370 DHH1 Tb927.10.3990 1 0.00125 CPSF73, SMN-Lt, U1A-Lt, Lsm3-Lt, SmD3-Lt, TSR1IP-Lt, U2AF35-Lt, SF1-Lt 90
Ribosomal biogenesis and assembly proteins
21 LmjF.15.1380 NOP58 Tb09.160.3820 8 1.28E-08 SMN-Lt, SmD3-Lt, U2AF35-Lt 55
22 LmjF.21.1760 CBF5 Tb927.10.170 5,4 0.00000576 NHP2-Lt, U2AF35-Lt 54
23 LmjF.15.1470 SNU13 Tb09.160.3670 2 0.0000017 SMN-Lt, NHP2-Lt, Lsm3-Lt, SmD3-Lt, U2AF35-Lt 55
24 LmjF.10.0210 SIK1/ NOP56 Tb927.8.3750 3 0.0000179 NHP2-Lt, U2AF35-Lt 55
25 LmjF.32.0750 NRBD2/P37 Tb11.01.5590 3 0.001042 TSR1IP-Lt, U2AF35-Lt 91
26 LmjF.33.1870 NOG1 Tb11.02.0620 3 0.00000422 U2AF35-Lt 92
Helicases
27 LmjF.22.1500 ATP-dependent DEAD/H RNA helicase, putative Tb927.6.740 3 0.000409 U2AF35-Lt GeneDB
28 LmjF.27.1300 hypothetical protein, conserved (p58) Tb11.46.0009 13 0.0000128 NHP2-Lt, TSR1IP-Lt, U2AF35-Lt GeneDB
Hypothetical proteins
29 LmjF.36.1580 hypothetical protein, conserved Tb927.10.6000 2 0.010697 SF1-Lt GeneDB
30 LmjF.28.2330 hypothetical protein, conserved Tb11.01.3480 1 0.000000199 PRP19-Tb, SmD3-Lt, 34
31 LmjF.18.0300 hypothetical protein, conserved Tb927.10.13800 1 0.007004 SmD3-Lt, 34
32 LmjF.35.5390 hypothetical protein, conserved Tb927.4.310 1 0.005636 TSR1IP-Lt, U2AF35-Lt, SF1-Lt GeneDB

L. tarentolae proteins were identified by mass spectroscopy as described in Materials and Methods. Each protein is described by its accession number (http://www.genedb.org), annotation, T. brucei homolog, numbers of peptides, other purifications revealing the same protein, and reference. Proteins were grouped into splicing factors, mRNA metabolism, ribosomal biogenesis and assembly proteins, helicases, and hypothetical proteins. The e-value reflects the probability of identification of the protein by MS data.