Skip to main content
. 2014 May 13;11(6):715–731. doi: 10.4161/rna.29143

Table 2. Mass spectrometric identification of TSR1IP purification products (L.t.).

  Protein category and accession number Annotation Trypanosoma brucei homolog Number of peptides e-value Purifications Reference(s) or source
Splicing factors
1 LmjF.34.0495 TSR1IP Tb927.10.2910 12 0.00000464 SF1-Lt 30
2 LmjF.07.0870 TSR1/RBSR1 Tb927.8.900 6 5.87E-07 NHP2-Lt, TSR1-Lt, U2AF35-Lt 29
3 LmjF.27.2100 TRRM1 Tb927.2.4710 2 0.005601 TSR1-Lt, U2AF65-Tb, U2AF35-Lt 28
4 LmjF.32.0400 ATP-dependent RNA helicase, putative Tb927.10.14550 6 1.42E-08 CPSF73, SMN-Lt, NHP2-Lt, PRP19-Tb, U1A-Lt, Lsm3-Lt, SmD3-Lt, TSR1-Lt, U2AF65-Tb, U2AF35-Lt, SF1-Lt 34
5 LmjF.07.0340 ATP-dependent DEAD/H RNA helicase, putative Tb927.8.1510 2 0.00000798 CPSF73, SMN-Lt, U1A-Lt, Lsm3-Lt, SmD3-Lt, TSR1-Lt, U2AF35-Lt, SF1-Lt 34
mRNA metabolism
6 LmjF.35.5040 PABP1 Tb09.211.0930 1 0.000261 NHP2-Lt, U1A-Lt, SmB-Tb,65 SmD1-Tb,38 SmD3-Lt, TSR1-Lt, U2AF35-Lt 82 , 83
7 LmjF.35.4130 PABP2 Tb09.211.2150 6 0.000178 NHP2-Lt, PRP19-Tb, SmB-Tb,65 SmD1-Tb,38 SmD3-Lt, TSR1-Lt, U2AF35-Lt 84
8 LmjF.25.0080 PABP ** 3 0.000073 NHP2-Lt, SmB-Tb,65 SmD1-Tb,38 SmD3-Lt, TSR1-Lt, U2AF35-Lt GeneDB
9 LmjF.35.2200 DRBD2 Tb09.211.4540 2 0.008095 NHP2-Lt, TSR1-Lt, U2AF35-Lt 85
10 LmjF.09.1120 MRP2 Tb11.01.4860 1 0.006042 TSR1-Lt, U2AF35-Lt 49 , 89
11 LmjF.19.1270 RNA binding protein, putative Tb927.10.15870 2 0.00000954   GeneDB
12 LmjF.35.0370 DHH1 Tb927.10.3990 1 0.002744 CPSF73, SMN-Lt, U1A-Lt, Lsm3-Lt, SmD3-Lt, TSR1-Lt, U2AF35-Lt, SF1-Lt 90
Ribosomal biogenesis and assembly proteins
13 LmjF.32.0750 NRBD2/P37 Tb11.01.5590 1 0.0000288 TSR1-Lt, U2AF35-Lt 91
Helicases
14 LmjF.27.1300 (p58) hypothetical protein, conserved Tb11.46.0009 2 0.000324 NHP2-Lt, TSR1-Lt, U2AF35-Lt GeneDB
Hypothetical proteins
15 LmjF.35.5390 hypothetical protein, conserved Tb927.4.310 1 0.005682 TSR1-Lt, U2AF35-Lt, SF1-Lt GeneDB
16 LmjF.35.4870 hypothetical protein, conserved Tb09.211.1230 1 0.0000148 SF1-Lt GeneDB
17 LmjF.08.0340 hypothetical protein, conserved Tb927.5.3330 1 0.007234 SF1-Lt GeneDB

L. tarentolae proteins were identified by mass spectroscopy as described in Materials and Methods. Each protein is identified by its accession number (http://www.genedb.org), annotation, T. brucei homolog, numbers of peptides, other purifications revealing the same protein, and reference. Proteins were grouped into splicing factors, mRNA metabolism, ribosomal biogenesis and assembly proteins, helicases and hypothetical proteins. The e-value reflects probability of identification of the protein by the MS data.