Figure 5. Treatment with C16 and C51 affects colocalization of MBNL1 with CUG RNA foci. Representative confocal images of RNA-FISH/IF of cultured human DM1 myoblasts that were either untreated or treated with DMSO (0.01%), C16 (0.5 μM), and C51 (30 μM). Treatment with C16 and C51 resulted in the dislodging of MBNL1 from faint nuclear CUGexp RNA foci and redistribution within the cells; DMSO had no such effect. Nuclei were stained with DAPI (blue); the CUGexp RNA foci are indicated by red fluorescence and MBNL1 by green fluorescence. Scale bar, 10 μm. For more images of RNA-FISH and MBNL1 immunofluorescence in DM1 fibroblasts, please refer to Figure S3A and B.