Table 2. Dissociation constants for TIA-1 RRM domains-RNA complexes by SPR.

Construct	RNA	KD (μM)
RRM2	U-rich 17mer	0.646 ± 0.005
	(ACUCC)x4	7.34 ± 0.07
	(GCUCC)x4	17.1 ± 0.1
	C-rich TIAR*	> 30
RRM3	U-rich 17mer	> 40
	(ACUCC)x4	> 100
	(GCUCC)x4	> 200
RRM23	U-rich 17mer	0.1042 ± 0.0007
	(ACUCC)x4	0.418 ± 0.001
	(GCUCC)x4	0.958 ± 0.002
	C-rich TIAR*	3.50 ± 0.01

^*The C-rich TIAR RNA oligo refers to the UUGCCACCUCCUGCUCCUGCCCAGA biological sequence of TIAR reported in reference 18.