Figure 1. Effects of HOTAIRM1 knockdown on growth and differentiation of ATRA-induced NB4 cells. (A) Quantitative RT-PCR measurement of HOTAIRM1 knockdown in NB4 cell lines transfected by lentiviral vectors expressing the indicated HOTAIRM1-targeting shRNAs. HOTAIRM1 RNA levels were determined at the indicated days of ATRA induction (mean ± SD; n = 3); (B) Growth curves shown as total cell counts at the indicated days of ATRA induction (mean ± SD, n = 3–9; * P < 0.05, two-way ANOVA Holm–Sidak post-hoc test); (C) Leukocyte differential counts of knockdown and control NB4 cells at day 4 of ATRA induction (mean ± SD, n = 3–9; * P < 0.05, two-way ANOVA Holm–Sidak post-hoc test).