FIG. 10.
Effect of Egr-1 antisense oligonucleotide on H. pylori-induced CD44 expression on AGS cells. AGS cells (5 × 105 cells/ml) were transfected with 5 μg of antisense Egr-1 oligonucleotide or Egr-1 sense sequence or were left untransfected. After an incubation for 8 h with H. pylori (6 × 108 CFU/ml), the cells were stained with a FITC-labeled anti-CD44 antibody (D2.1 ascites; shaded peaks) or a FITC-labeled isotype control antibody (anti-IE; unshaded peaks). (A) Resting untransfected AGS cells; (B) AGS cells transfected with Egr-1 sense sequence; (C) AGS cells transfected with Egr-1 antisense oligonucleotide; (D) AGS incubated with H. pylori; (E) H. pylori-induced CD44 expression on AGS cells transfected with Egr-1 sense sequence; (F) downregulation of H. pylori-induced CD44 expression on AGS cells by Egr-1 antisense oligonucleotide. Each experiment was repeated three times with similar results, and a representative result is shown.