FIG. 8.

Regulation of H. pylori CD95L promoter activity by Egr-1. (A) Schematic representation of the luciferase reporter of the wild-type CD95L promoter from −860 to +100, with or without a mutation at the −680, −180, and −120 Egr-1 binding sites. (B) CD95L constructs were transfected into AGS cells as equivalent amounts of plasmids bearing wild-type CD95L and mutant CD95L promoters. After stimulation with H. pylori (6 × 10⁸ CFU/ml) for 24 h, cell lysates were prepared and assayed for luciferase activity. Normalized luciferase activity was defined as the activity of the luciferase reporter gene relative to the activity of the β-galactosidase plasmid promoter. All transfection assays were performed three times with similar results, and the results for one experiment are shown as means ± SD.