FIG. 9.
Effect of Egr-1 antisense oligonucleotide on H. pylori-induced Egr-1 expression. (A) AGS cells were transfected with 5 μg of antisense Egr-1 oligonucleotide (Egr-1/PcDNA3.1+/Zeo) or the Egr-1 sense sequence or were left alone for 48 h. After an incubation of AGS cells with H. pylori (6 × 108 CFU/ml) for 8 h, total cell lysates were prepared and analyzed for Egr-1 protein expression by immunoblotting. Lanes 1 and 4, nontransfected AGS cells; lanes 2 and 5, AGS cells transfected with Egr-1 sense sequence; lanes 3 and 6, AGS cells transfected with Egr-1 antisense oligonucleotide. (B) Dose-dependent inhibition of H. pylori-induced Egr-1 expression in AGS cells by Egr-1 antisense oligonucleotide. Three independent experiments were performed with similar results, and a representative gel is shown. S, Egr-1 sense; AS Egr-1, antisense Egr-1.