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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Prostate. 2014 Jul 7;74(12):1209–1221. doi: 10.1002/pros.22837

Fig. 5.

Fig. 5

ATG5 knockdown enhances HNK-induced apoptosis. A: Immunoblotting for ATG5-ATG12 and LC3BII in PC-3 cells. Numbers on top of bands are fold change in ATG5-ATG12 protein expression relative to DMSO-treated control siRNA-transfected cells (first lane). B: Viability of PC-3 cells. C: Detection of apoptosis in PC-3 cells. For data shown in panels AC, PC-3 cells were transiently transfected with control siRNA or ATG5 siRNA followed by 24 h treatment with DMSO or 40 μM HNK. Results shown are mean ± SD (n = 3). *Statistically significant between the indicated groups by one-way ANOVA followed by Bonferroni’s multiple comparison test.