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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Prostate. 2014 Jul 7;74(12):1209–1221. doi: 10.1002/pros.22837

Fig. 7.

Fig. 7

HNK causes ROS production in prostate cancer cells. A: Representative EPR spectra using CMH (1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine) as a spin probe, and quantitation of EPR signal intensity from PC-3 cells treated with DMSO or 40 μM HNK for 4 h. Combined results from two independent experiments are shown as mean ± SD (n = 6). Statistical significance was determined by Student t test. B: Representative flow histograms and quantitation of MitoSOX Red fluorescence in PC-3 cells treated with DMSO or 40 μM HNK for 2 h or 4 h. Results shown are mean ± SD (n = 3). Statistical significance was determined by Student t test. C: Representative microscopic images showing MitoSOX Red fluorescence in PC-3 cells after 2 h or 4 h treatment with DMSO or 40 μM HNK (100× objective magnification).