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U937 cells express TLR2 and TLR4. Untreated monocytic and PMA-differentiated U937 cells were labeled with anti-TLR (gray bars, TLR2; black bars, TLR4) or isotype antibodies (white bars). Binding was quantified by measuring mean channel fluorescence (± standard errors of the means) by laser scanning cytometry. Assays were performed in duplicate or triplicate (n = 4).
