TABLE 3.
Cytokine levels in spleen homogenates
| Cytokine | Mean cytokine level (pg/ml) ± SDa in:
|
||
|---|---|---|---|
| C57BL/6j mice | IL-6−/− mice | IL-6−/− mice + rIL-6 | |
| TNF-α | 16 ± 15 | 60 ± 89 | 22 ± 16 |
| IFN-γ | 83 ± 23* | 168 ± 41 | 65 ± 26 |
| MCP-1 | 35 ± 12* | 130 ± 81 | 46 ± 34 |
| IL-10 | 29 ± 4 | 19 ± 17 | 22 ± 5 |
| IL-6 | 58 ± 18 | ND | ND |
| IL-12p70 | ND | ND | ND |
a
Cytokine levels present in the spleen homogenate of infected mice were determined by CBA. ND, no detectable cytokine. Asterisks indicate a statistically significant result when C57BL/6j mice are compared to IL-6−/− mice. Samples were compared by using a Mann-Whitney test (P = 0.007).