Data are the proportions of youth eligible to take the ACASI who received chlamydia/gonorrhea testing (urine, cervical, or penile) each month (red bars), overall proportion tested that period (blue line), and Bonferroni-adjusted 95% confidence interval (CI) limits for testing proportions in each period (blue shading). Pink bars represent partial months. In the ACASI period the blue line represents before and after the nursing standing order was available on September 1, 2011. It excludes patient visits with ED chief complaints that are genitourinary or gynecologic, and chief complaints of abdominal/pelvic pain in women. In the control period, 2.8% (91/3281; Bonferroni-adjusted 95% CI [2.1-3.6]) received testing. During the initial education only period, 2.7% (22/820; Bonferroni-adjusted 95% CI [1.6-4.5]) received testing. After ACASI introduction, 10.4% (227/2188; Bonferroni-adjusted 95% CI 8.9-12.1]) received testing. After ACASI withdrawal, 4.3% (33/760; Bonferroni-adjusted 95% CI [2.8-6.6]) received testing.