FIG. 3.
Expression of total Bcl-2 protein. B cells were cultured for 15 h, as indicated, in medium alone or in the presence of 5 μg of LPS/ml, 10 nM STR, or LPS plus STR. (A) The presence of Bcl-2 in total cell lysates obtained from 2 × 106 cells was detected by Western blotting. (B) Relative Bcl-2 protein levels were determined by densitometry and reported as fold changes compared to control ex vivo B cells at the initiation of cultures. Data are the means ± SD of three independent experiments.