Table 1.
Average concentration of Fe, Cu, Zn and Mn in large regions of the brain
| μg g−1wet weight | Control | WD | %** change | p- value |
|---|---|---|---|---|
| Entire section | ||||
| Fe | 14.1 ± 2.5 | 14.1 ± 1.0 | .9 | |
| Cu | 4.2 ± 1.4 | 6.7 ± 1.5 | +61% | .0007 |
| Zn | 13.2 ± 3.1 | 15.8 ± 1.7 | +19% | .07 |
| Mn | 0.25 ± 0.07 | 0.26 ± 0.05 | .4 | |
| Cortex (grey matter) | ||||
| Fe | 12.4 ± 2.2 | 12.1 ± 0.8 | .7 | |
| Cu | 2.7 ± 0.7 | 5.5 ± 1.2 | +108% | .00004 |
| Zn | 13.9 ± 3.0 | 17.7 ± 0.8 | +28% | .008 |
| Mn | 0.23 ± 0.06 | 0.26 ± 0.04 | .2 | |
| Corpus callosum (white matter)* | ||||
| Fe | 7.8 ± 1.1 | 7.6 ± 0.6 | .6 | |
| Cu | 1.5 ± 0.5 | 2.9 ± 0.8 | +102% | .0004 |
| Zn | 8.2 ± 2.1 | 9.5 ± 1.4 | .2 | |
| Mn | 0.11 ± 0.03 | 0.13 ± 0.04 | .2 | |
| Cerebellum, Cu rich regions lobules 4–5, 8-10 | ||||
| Fe | 17.4 ± 3.8 | 17.4 ± 2.1 | 1 | |
| Cu | 6.6 ± 1.6 | 10.9 ± 1.7 | +64% | .0001 |
| Zn | 13.0 ± 2.9 | 16.0 ± 2.0 | +23% | .03 |
| Mn | 0.28 ± 0.09 | 0.28 ± 0.04 | .9 | |
| Cerebellum Cu poor regions (other) | ||||
| Fe | 17.8 ± 3.5 | 16.3 ± 3.8 | ||
| Cu | 3.8 ± 0.9 | 7.5 ± 1.6 | +95% | .0002 |
| Zn | 12.3 ± 2.7 | 13.8 ± 3.1 | .3 | |
| Mn | 0.30 ± 0.08 | 0.27 ± 0.06 | 1 | |
| Cerebellar white matter* | ||||
| Fe | 8.4 ± 1.9 | 6.9 ± 1.9 | .1 | |
| Cu | 1.7 ± 0.3 | 4.0 ± 1.2 | +132% | .0004 |
| Zn | 6.8 ± 1.4 | 7.9 ± 1.2 | .1 | |
| Mn | 0.15 ± 0.04 | 0.13 ± 0.06 | .9 | |
| Colliculus superior | ||||
| Fe | 13.8 ± 2.2 | 13.3 ± 1.1 | .7 | |
| Cu | 3.3 ± 0.7 | 6.1 ± 0.5 | +85% | .0005 |
| Zn | 9.8 ± 2.0 | 11.4 ± 0.4 | .08 | |
| Mn | 0.31 ± 0.07 | 0.34 ± 0.07 | .3 | |
| Colliculus inferior | ||||
| Fe | 15.7 ± 3.4 | 12.1 ± 1.5 | .05 | |
| Cu | 3.6 ± 0.9 | 5.4 ± 0.7 | +49% | .003 |
| Zn | 9.5 ± 2.1 | 11.2 ± 1.7 | .1 | |
| Mn | 0.36 ± 0.10 | 0.35 ± 0.09 | .06 | |
| Periventricular regions | ||||
| Lateral ventricules | ||||
| Cu | 39 ± 17 | 17 ± 3 | −56% | .01 |
| Third ventricule | ||||
| Cu | 50 ± 28 | 22 ± 4 | −57% | .02 |
| Fourth ventricule | ||||
| Cu | 67 ± 22 | 19 ± 5 | −71% | .0004 |
Concentrations of Fe, Cu, Zn, Mn (mean ± SD) measured by imaging LA-ICP-MS in regions of interest in the brain of 8 controls, and 9 WD mice, both on a SVJ129 background. Of each animal, one section was measured whereby each hemisphere was measured in an independent run with separate calibration standard. SD and heteroskedastic t-tests were calculated over the 8 and 9 individual bi-hemispheric averages.
*white matter borders were delineated to minimize spillover from neighbour regions.
**indicated, if > +10% or < −10% and p < .05.