Table 5.
List of antibodies used
| Antibody | Source | Dilution | Antigen retrieval |
|---|---|---|---|
| CK7 | Mouse monoclonal; Dako, Glostrup, Denmark | 1:50 | Citrate buffer, pH 6.0 |
| EpCAM | Mouse monoclonal; Dako, Glostrup, Denmark | 1:200 | Citrate buffer, pH 6.0 |
| CD133 | Rabbit monoclonal; Cell Signaling Technology, Danvers, MA | 1:100 | Citrate buffer, pH 6.0 |
| CD117 (c-kit) | Rabbit polyclonal; Dako, Glostrup, Denmark | 1:400 | EDTA buffer, PH 8.0 |
| OV6 | Mouse monoclonal; R & D Systems, Emeryville, CA | 1:40 | EDTA buffer, PH 8.0 |
| PCNA | Mouse monoclonal; Cell Signaling Technology, Danvers, MA | 1:4000 | Citrate buffer, pH 6.0 |
CK7 cytokeratin 7, EpCAM epithelial cell adhesion molecule , CD133 cluster of differentiation (CD)133, CD117 (c-kit) mast/stem cell growth factor receptor, OV6 oval cell marker, PCNA proliferating cell nuclear antigen.