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. 2014 Aug 20;15(1):697. doi: 10.1186/1471-2164-15-697

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© Lopez-Gomollon et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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sRNAs from F. cylindrus and E. huxleyi. Northern blot validation of F. cylindrus (a) and E. huxleyi (b) candidates and their expression in T. pseudonana (Tp), P. tricornutum (Pt), F. cylindrus (Fc), and E. huxleyi (Eh). ZR small RNA ladder (left) and EtBr gel (bottom) are provided for band sizing and to show equal loading of the above RNAs. (c) and (d), examples of F. cylindrus validated tRNAs producing sRNA sequences. The top visualisation represents the mapping pattern of sRNAs stacked up against the y-axis mapped to the tRNA sequence on the x-axis. The secondary structure of the tRNA is shown in dot-bracket notation above the x-axis. The middle graph shows the size class distribution of all sRNAs that map to the tRNA and the bottom schematic shows the folded tRNA with its most abundant sRNA from the library highlighted in red.