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SPR analysis of binding of Ia to Ib immobilized on a dextran matrix flow cell surface by primary amine coupling. Injections of Ia (at the indicated concentrations) in HBSS were made onto the Ib oligomer surfaces. The binding of Ia was recorded in real time. Injections were done at 10 μl/min for 170 s, followed by HBSS alone. The experiments were repeated three times, and results for a representative experiment are shown.
