TABLE 3.
Inhibitory effect of Ia deletion mutants on cytotoxic activity induced by Ia plus Iba
| Deletion mutant | Cell rounding with indicated dose of mutant (μg/ml)
|
||||||
|---|---|---|---|---|---|---|---|
| 0 | 0.05 | 0.1 | 0.5 | 1 | 5 | 10 | |
| Ia1-210 | +++ | +++ | ++ | + | − | − | − |
| Ia21-210 | +++ | +++ | ++ | + | − | − | − |
| Ia101-210 | +++ | +++ | +++ | ++ | + | − | − |
| Ia151-210 | +++ | +++ | +++ | +++ | ++ | + | − |
| Ia211-413 | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
a
Confluent monolayers of MDCK cells (2 × 106 cells) were exposed to various doses of variant Ia deletion mutants in the presence of 10 ng of wild-type Ia/ml and 500 ng of Ib/ml at 37°C. Cell rounding was recorded after 8 h. Cell rounding activity was scored as follows: +++, 80 to 100 % rounding; ++, 50 to 80% rounding; +, 20 to 40% rounding; −, no rounding. The experiments were repeated three times, and data from a representative experiment are shown.