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. 2014 Aug 24;7:391. doi: 10.1186/1756-3305-7-391

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© Dada et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PCoA of unweighted UniFrac distances between samples analyzed by TTGE. Principal coordinate analysis of unweighted UniFrac distances between Ae. aegypti larvae and water from mosquito positive and negative containers. Distinct clusters of larval samples (red) and mosquito positive water samples (blue) are captured on all three PCoA plots. Mosquito negative water samples (orange) do not show any distinct clusters on any of the plots. Each axis show percentage of variation explained. Each data point consists of a central point surrounded by ellipsoids that indicate variation in UniFrac distances from rarefaction. This demonstrates that the clustering pattern holds up to subsampling.